Figure 3. IgG subclass and antibody mediated effector functions do not influence vaccine efficacy.

Mice (n=6/group) were passively immunized with recombinant mAb expressed as IgG₁, IgG_2a, or IgG_2a LALA-PG (mutation which prevents binding to FcγRI-IV). One day later, serum was collected to measure A) antibody concentration via biolayer interferometry. Mice were then challenged with 2.25 mg/kg oxycodone and B) blood and C) brain were collected 30 minutes later to determine opioid concentration by LC-MS/MS. Data are mean±SEM. Statistical analysis performed via one-way ANOVA with Tukey’s multiple comparisons post hoc test. Two independent experiments were performed, data shown are representative from one experiment. Stars directly over columns indicate significance compared to control. Statistical symbols: ** p<0.01, *** p<0.001, **** p<0.0001