Table 2.
Primers used for the detection of virulence genes in E. coli and S. aureus.
| Germs | Genes | Sequences (5′->3′) | Sizes (bp) | References |
|---|---|---|---|---|
| E. coli | stx1 | F: ACACTGGATGATCTCAGTGG R: CGTAATCCCCCTCCATTATG |
614 | [17] |
| stx2 | F: CCATGACAACGGACAGCAGTT R: CCTGTCAACTGAGCAGCACTTTG |
779 | [17] | |
| LT | F: GCGACAAATTATACCGTGCT R: CCGAATTCTGTTATATATGT |
708 | [18] | |
| ST | F: CTGTATTTGTCTTTTTCACCT R: GCACCCGGTACAAGCAGGAT |
182 | [18] | |
|
| ||||
| S. aureus | sea | F: GGTTATCAATGTGCGGGTGG R: CGGCACTTTTTTCTCTTCGG |
102 | [15] |
| seb | F: GTATGGTGGTGTAACTGAGC R: CCAAATAGTGACGAGTTAGG |
164 | [15] | |
| sec | F: AGATGAAGTAGTTGATGTGTATGG R: CACACTTTTAGAATCAACCG |
451 | [15] | |
| sed | F: CCAATAATAGGAGAAAATAAAAG R: ATTGGTATTTTTTTTCGTTC |
278 | [15] | |
| see | F: AGGTTTTTTCACAGGTCATCC R: CTTTTTTTTCTTCGGTCAATC |
209 | [15] | |
stx1, Shiga toxin 1; stx2, Shiga toxin 2; LT, heat-labile toxin; ST, heat-stable toxin; sea, seb, sec, sed, and see: Staphylococcal enterotoxins, a–e.