Table 3.

Virulence gene amplification program.

Amplification steps	Amplification conditions (temperature and weather)
	stx1/stx2	ST/LT	sea/seb/sec/sec/sed/see
Initial denaturation	94°C/5 min	94°C/5 min	94°C/5 min
Cyclic denaturation	94°C/30 s	94°C/45 s	94°C/2 min
Hybridization	56°C/30 s	60°C/1 min	57°C/2 min
Cyclic elongation	72°C/30 s	72°C/1 min	72°C/1 min
Final elongation	72°C/10 min	72°C/7 min	72°C/7 min
Number of cycles	30	35	35