Table 5.
Primers used for the detection of resistance genes in E. coli, Salmonella spp., and S. aureus.
| Germs | Genes | Sequences (5′->3′) | Sizes (bp) | References |
|---|---|---|---|---|
| E. coli and Salmonella spp. | bla TEM | F: ATGAGTATTCAACATTTCCGTG R: TTACCAATGCTTAATCAGTGAG |
840 | [19] |
| bla SHV | F: TTTATGGCGTTACCTTTGACC R: ATTTGTCGCTTCTTTACTCGC |
1051 | [20] | |
| bla CTX –M | F: GGTTAAAAAATCACTGCGTC R: TTGGTGACGATTTTAGCCGC |
863 | [21] | |
| qnrA | F: GATAAAGTTTTTCAGCAAGAGG R: ATCCAGATCGGCAAAGGTTA |
543 | [21] | |
| qnrB | F: GACAGAAACAGGTTCACCGGT R: CAAGACGTTCCAGGAGCAACG |
469 | [21] | |
| qnrS | F: ACGACATTCGTCAACTGCAA R: TAAATTGGCAACCTGTAGGC |
417 | [21] | |
|
| ||||
| S. aureus | mecA | F: TGCTATCCACCCTCAAACAGG R: AACGTTGTAACCACCCCAAGA |
286 | [22] |
blaTEM, blaSHV, and blaCTX-M: beta-lactam resistance genes; qnrA, qnrB, and qnrS: fluoroquinolone resistance marker genes; mecA: meticillin resistance; E. coli, Escherichia coli; S. aureus, Staphylococcus aureus.