Table 6.

Resistance gene amplification conditions.

Amplification steps	Temperature condition/times
	blaCTX-M, blaTEM, blaSHV	qnrA, qnrB, qnrS	mecA
Initial denaturation	94°C/5 min	95°C/5 min	94°C/4 min
Cyclic denaturation	94°C/1 min	95°C/30 s	94°C/30 s
Hybridization	60°C/1 min	60°C/30 s	60°C/1 min
Cyclic elongation	72°C/1 min	72°C/1 min	72°C/2 min
Final elongation	72°C/7 min	72°C/10 min	72°C/4 min
Number of cycles	(30)	(30)	(35)