Fig. 5. The JM domain of VAMP2 enables αSYN phase separation.

a, Schematic of VAMP2 with the respective NT peptide and JMD peptide. b, Evaluation of αSYN phase separation in the presence of 150 µM peptide and crowding with 15% PEG 8000, 30 min after PEG addition (t_{30 min}), 40 µM αSYN. c, Quantification of turbidity measurements at t_{30 min}. Data are mean ± s.d. from three biological repeats; n indicates biological repeats. Unpaired two-tailed t-test. d, Schematic of VAMP2 with the respective N-terminal long peptides (NT long peptide 1 and 2), the native JMD long peptide and the SNARE–JMD long peptide. e, Evaluation of αSYN phase separation in the presence of 150 µM peptide and crowding with 15% PEG 8000, 30 min after PEG addition (t_{30 min}), 40 µM αSYN. f, Quantification of turbidity measurements at t_{30 min}. Data are mean ± s.d. from three biological repeats; n indicates biological repeats. One-way ANOVA with Dunnett’s multiple comparison test.

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