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. 2000 Dec;74(23):11240–11246. doi: 10.1128/jvi.74.23.11240-11246.2000

FIG. 5.

FIG. 5

Ammonium sulfate fractionation of FB extract containing recombinant XcGV-MMP expressed by BmMMPCysPD. (A) Proteinase activity of the FB fractions collected from ammonium sulfate precipitation. 1, FB extract; 2, 0 to 20% saturated ammonium sulfate fraction; 3, 20 to 40% saturated ammonium sulfate fraction; 4, 40 to 60% saturated ammonium sulfate fraction; 5, 60 to 80% saturated ammonium sulfate fraction. (B) Western blot analysis of the ammonium sulfate fractions. The sample numbers in panel A correspond to the sample numbers in panel B. The relative intensities of the bands reacting with MMP antibody were as follows: 1, 185.77; 2, 92.93; 3, 64.97; 4, 47.67; and 5, 19.48 (for the 54-kDa band); and 1, 153.57; 2, 90.11; 3, 13.02; 4, 2.97; and 5, 0 (for the 30-kDa band). FB extract was prepared from FB collected from BmMMPCysPD-infected B. mori larvae 5 days p.i.