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. 2024 Aug 13;7:985. doi: 10.1038/s42003-024-06674-9

Fig. 5. Impact of PCSK9 Inhibition on Lipid Accumulation, Lipid Peroxidation, and Cellular growth.

Fig. 5

a Lipid level was measure by lipid accumulation specific bodipy reagent. Inhibition of PCSK9 was associated with an increase in lipid accumulation. b, c Lipid peroxidation was measured by lipid peroxidation specific bodipy and lipid peroxidation product MDA (n = 3). In compared to untreated (UT), cigarette smoke extract (CSE) induced a higher level of lipid peroxidation in cells in the PCSK9-suppressed PBEC. d Cell cycle was analyzed by propidium iodide staining. PCSK9 inhibition affected cell cycle, primarily affecting the G2/M phase. e LDH assay was performed to determine cell death. CSE-induced cell death was notably higher in PCSK9-silenced cells compared to control siRNA transfected cells (n = 6). f,g In response to the staurosporine, PCSK9 inhibition induced apoptosis and an increase in caspase 3 activation (n = 3). Error bars represent median interquartile range. p-value ≤ 0.05 was considered statistically significant.