Table 2.
Urine steroid metabolome results for this patient. Results corrected for concentration by measurement of urine creatinine (patient’s creatinine is 1.9 mmol/L).
| Steroids | Abbreviations | Chem Spider ID | Retention time (minute) | Target ion (m/z) | Quantitative Ion (m/z) | Concentration |
||
|---|---|---|---|---|---|---|---|---|
| µmol/L | µmol /mmol creatinine | RI*µmol/mmol | ||||||
| Internal Standard (androstanol) | ISTD | 13.38 | 258 | 243 | ||||
| Androsterone | A | 5668 | 16.96 | 360 | 270 | 0.3 | 0.2 | <0.2 |
| Etiocholanolone | E | 5669 | 17.18 | 360 | 270 | 0.6 | 0.3 | <0.1 |
| Dehydroepiandrosterone | DHEA | 5670 | 18.12 | 358 | 268 | 0.5 | 0.3 | <1.3 |
| 11-Ketoandrosterone | 11-KA | 18.54 | 315 | 300 | 0.1 | 0.1 | <0.2 | |
| 11-OH Androsterone | 11-OH A | 20,171,894 | 19.57 | 358 | 448 | 0.2 | 0.1 | <1.1 |
| 17-hydroxypregnanolone** | 17-OHPN | 19.61 | 296, 386, 476 | Present | ||||
| 11-OH Etiocholanolone | 11-OH E | 92020 | 19.81 | 358 | 448 | 0.1 | 0.1 | <0.2 |
| Pregnanediol | PD | 190585 | 20.51 | 269 | 284 | 4.6 | 2.4 | <0.2 |
| Pregnanetriol | PT | 92121 | 20.88 | 255 | 435 | 2.1 | 1.1 | <0.2 |
| Androstenetriol | A’3 | 21.17 | 432 | 342 | 13.4 | 7.1 | <5.8 | |
| Tetrahydro-deoxycortisol | THS | 29788211 | 21.86 | 474 | 564 | 23.1 | 12.1 | <0.2 |
| Hexahydro-compound S (double peak)** | HHS | 23.16& 23.33 | 255, 345, 435 | Present | ||||
| Tetrahydrocortisone | THE | 29788209 | 23.42 | 488 | 578 | 2.5 | 1.3 | 0.2–5.5 |
| Tetrahydrocortisol | THF | 5665 | 23.93 | 472 | 562 | 1.0 | 0.5 | <0.3 |
| Allo-Tetrahydrocortisol | Allo-THF | 83726 | 24.09 | 472 | 562 | 1.5 | 0.8 | <1.9 |
| Ratios | ||||||||
| Steroids | Result | RI | ||||||
| THS/(THE + THF + Allo-THF) | 2.28 | <0.3 | ||||||
*
The reference intervals were developed in house from 26 healthy (not in-hospital) Vietnamese children from one-month to 12-months old.
**
Steroid metabolites were qualitatively assessed based on m/z ions and relative retention time.