Table 3.
The intra- and inter-operator reproducibility of loading the Mitra devices with blood and the intra- and inter-day reproducibility of extraction, digestion, and LC-MS/MS measurement.
| Protein | Peptide | Operator reproducibility |
Workflow reproducibility |
||||
|---|---|---|---|---|---|---|---|
| %CV intra | %CV inter | %CV total | %CV intra | %CV inter | %CV total | ||
| n = 6 | o = 5 | n = 5 | d = 5 | ||||
| apoA-I | THLAPYSDELR | 5.2 | 8.2 | 9.7 | 6.0 | 0.0 | 6.0 |
| apoB | FPEVDVLTK | – | – | – | 3.8 | 0.0 | 3.8 |
| GFEPTLEALFGK | – | – | – | 8.1 | 9.7 | 12.6 | |
| apoC-I | TPDVSSALDK | 4.9 | 7.8 | 9.2 | 6.6 | 2.9 | 7.2 |
| apoC-III | GWVTDGFSSLK | 4.7 | 6.2 | 7.8 | 6.2 | 5.8 | 8.5 |
| apoE | SELEEQLTPVAEETR | 6.5 | 6.9 | 9.5 | 10.4 | 0.0 | 10.4 |
| AATVGSLAGQPLQER | 4.2 | 8.0 | 9.0 | 6.1 | 3.2 | 6.9 | |
| LGPLVEQGR | 5.7 | 8.4 | 10.2 | 4.6 | 2.7 | 5.4 | |
| HSA | DDNPNLPR | – | – | – | 3.6 | 2.4 | 4.3 |
| LVNEVTEFAK | 4.5 | 8.8 | 9.9 | 3.2 | 1.0 | 3.4 | |
Within-operator and within-day%CV are expressed as the average%CV of n replicates over o operators or d days, respectively. The total%CV (%CVintra+inter) is calculated as (CVintra2 + CVinter2)1/2, where CVinter is calculated as the%CV of the individual or daily mean (B) corrected for%CVintra (CVinter = (B2 − CVintra2/n)1/2.