FIG. 3.

Intracellular localization of chimeric proteins. (A) HeLa cells expressing chimeric proteins were permeabilized and immunostained with anti-US3 antibodies, followed by FITC-conjugated goat anti-rabbit IgG. (B) Cells were fixed, permeabilized with Triton X-100 (lower panel) or not permeabilized (upper panel), and immunostained with anti-CD4 MAb OKT4, followed by FITC-conjugated anti-mouse IgG. Shown are representative fields from multiple independent transfections. Transfection efficiencies were generally 30 to 50%.