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. 2024 Jul 31;14:1425367. doi: 10.3389/fcimb.2024.1425367

Figure 2.

Figure 2

H. pylori induces the up-regulation of PGRN in gastric epithelial cells. (A) Enrichment plots of gene expression signatures for H. pylori infection according to PGRN mRNA expression in a GSEA analysis of TCGA. (B) Immunohistochemistry staining of PGRN in human normal, superficial gastritis and atrophic gastritis tissues. Scale bars, 20 μm. (C) qRT-PCR analysis of PGRN mRNA expression level in H. pylori-negative (n=19) and H. pylori-positive (n=24) chronic gastritis tissue samples. (D) Western blot of PGRN in in BGC-823 and GES-1 cells infected with H. pylori (MOI = 100) for 0, 6, 12 and 24 h. (E) Immunohistochemistry staining of PGRN in H. pylori-negative (n=11) and H. pylori-positive (n=32) GC tissues. Scale bars, 20 μm. (F) Immunohistochemistry staining of PGRN in containing H. pylori-negative and H. pylori- positive gastric tissues of mice. Scale bars, 50 μm (20X), 20 μm (40X). (G) Western blot of PGRN in H. pylori-negative and H. pylori-positive gastric tissues of mice. (*P<0.05, **P<0.01, ***P<0.001).