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. Author manuscript; available in PMC: 2024 Aug 14.
Published in final edited form as: Biotechnol Bioeng. 2016 Mar 6;113(9):2033–2040. doi: 10.1002/bit.25961

Figure 6.

Figure 6.

Phage display identified peptides specific to the VSV-G protein on the lentivirus. Specific binding of lentivirus to phage display peptides. PEG hydrogels were functionalized with 2.5 mM RGD and 2.5 mM of the synthesized phage display peptides conjugated with a 5 kDa PEG linker (n= 4). HLKHTHNTHYKTCG (“HLK”), HWKPHSNLHLSRCG (“HWK”), STQHHHHSKQSRCG (“STQ”), and WPGHHNHSMKHKCG (“WPG”). RGD and 30–70 kDa PLL (0.45mg/mL) were also functionalized to the PEG linker to serve as negative and positive controls, respectively. Lentivirus was incubated with the hydrogels for 3 h followed by washing to remove non-binding virus. HT1080cellswere seeded on the hydrogels and luciferase activity was assayed 72h later. Fold increase in GLuc expression relative to RGD control. Significant differences between RGD and PLL are denoted by an asterisk (P < 0.05).