Figure 1 .

Expression pattern of UBE2C

(A) The mRNA expressions of UBE2C and myogenic markers were measured in the longissimus dorsi of mice at five developmental stages. GAPDH was used as an internal control for normalization. E denotes the embryonic period, while P indicates post-birth. (B) The mRNA levels of UBE2C and myogenic markers were evaluated at five time points during C2C12 differentiation. (C) The protein expression levels of UBE2C and myogenic markers were evaluated at five time points during C2C12 differentiation (left). Grayscale scanning for quantifying protein expression (right). The cells were cultured in growth medium (GM) at sub-confluent densities. When the cells reached 100% confluence, it was defined as day 0 (D0), and the growth medium was changed to differentiation medium. Data are presented as the mean±SEM ( n=3 per group).