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. 2024 Apr 29;56(7):1065–1071. doi: 10.3724/abbs.2024062

Figure 2 .

Figure 2

UBE2C is essential for C2C12 differentiation

(A) The mRNA expression levels of UBE2C, MyoD, MyoG, and MyHC were quantified using qPCR one day after the induction of differentiation. (B) Western blot analysis was used to determine the protein expression levels of UBE2C, MyoG, and MyHC (left panel). Greyscale scanning of the western blots is shown in the right panel, with GAPDH serving as the loading control. (C) Immunofluorescence staining for MyoG was performed on C2C12 cells one day after induction of differentiation (left panel), and the percentage of MyoG-positive cells was calculated (right panel). (E) Western blot analysis was utilized to assess the protein expression levels of UBE2C, MyoG, and MyHC in cells overexpressing UBE2C (left panel). Greyscale scanning of the western blots is shown in the right panel. Immunofluorescence staining for MyHC was conducted three days after induction of differentiation in cells with UBE2C knockdown (D) or overexpression (F). The nuclei were counterstained with DAPI. Scale bar: 200 μm. Data are presented as the mean±SEM, n=3 per group. * P<0.05, ** P<0.01, *** P<0.001, and ns indicates no significant difference.