Figure 4 .

UBE2C knockdown restrains skeletal muscle regeneration

(A) The mouse model of TA tissue injury is illustrated in the schematic diagram. (B,C) After 7 days, the mRNA (B) and protein (C) expression levels of the given genes were evaluated in mice subjected to TA treatment with CTX. (D) The protein expression level of MyHC was assessed after 14 days of CTX treatment. (E) H&E staining was conducted on TA samples harvested at 3 d and 14 d after CTX treatment (left panel), and the proportion of muscle fibers with two or more central nuclei were quantified (right panel). Scale bar: 100 μm. Data are presented as the mean±SEM, n=3 per group. * P<0.05, and ** P<0.01.