Table 1. Specific activities of the mutants compared with wild-type enzyme and the two forms of the primitive dehalogenase in cell-free extracta.
| DBEb (pH 8.2) U/mg | DBEb (pH 7.5) U/mg | DCEb (pH 8.2) U/mg | BBb (pH 8.2) U/mg | DBE/BB ratio | |
|---|---|---|---|---|---|
| Wild type |
2.41 |
2.06 |
2.92 |
0.60 |
4.0 |
| DhlAS |
1.30 |
1.13 |
−c |
0.51 |
2.6 |
| DhlAT |
1.31 |
1.06 |
− |
0.52 |
2.5 |
| 2A2 |
2.17 |
1.43 |
− |
0.50 |
4.3 |
| 10D7 |
6.88 |
6.50 |
0.21 |
1.68 |
4.1 |
| D2 |
4.35 |
4.12 |
0.11 |
1.06 |
4.1 |
| D8 |
4.78 |
4.28 |
0.16 |
0.95 |
5.0 |
| 1C12 |
4.75 |
3.80 |
0.46 |
0.95 |
5.0 |
| 3B2 |
5.63 |
5.09 |
0.32 |
1.13 |
5.0 |
| 3C11 |
3.96 |
3.49 |
0.12 |
0.58 |
6.8 |
| 2C7 |
2.00 |
2.03 |
− |
0.59 |
3.4 |
| 3D3 |
3.30 |
3.03 |
− |
0.87 |
3.8 |
| 3F9 | 1.95 | 2.23 | − | 0.86 | 2.3 |
aAnalysis with SDS−PAGE confirmed that expression levels were similar.
bSubstrate concentrations were 5 mM.
c−, Not detectable/<0.02 U/mg.