FIG. 3.

EMSA demonstrates that Gal4(DBD)-p30^II binds Gal4 DNA element in a dose-dependent manner. (A) EMSA using nuclear extracts obtained from HeLa-tat cells 48 h posttransfection with p30^II-Gal4-pCRG4-11 as described in the text. The DNA oligonucleotide probe containing tandem Gal4-DNA-binding elements was labeled with γ-³²P by T4 kinase. The amounts of nuclear lysates and presence of anti-Gal4 serum (lane 10) and competitors (Comp.) (lanes 11 and 12) are indicated at the top of the gel. Free probe, probe complexes, and shifted bands are represented in panel B.