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. 2024 Jul 2;15(8):e01316-24. doi: 10.1128/mbio.01316-24

Fig 2.

An illustration displays fold changes in gene expression of various cell type markers, immunofluorescence staining and quantification comparing expression of SI, MUC2, and CHGA-positive cells in infant and adult enteroid monolayers.

Cell type composition varies between differentiated infant and adult HIE monolayers on transwells. (A) RNAseq transcript expression for select markers of absorptive (enterocytes) and secretory cells (goblet cells, enteroendocrine cells, Paneth cells, and tuft cells). Data represent mean values and are expressed as a Log2-fold change of gene expression in infant over adult HIEs. Red bars = upregulated in infant HIEs; blue bars = downregulated in infant HIEs. (B) Representative confocal images from four independent experiments, with each experiment including the three infant and three adult HIE lines. Top panel: enterocytes stained for expression of sucrase isomaltase (SI, red), middle panel: goblet cells (Muc2, green), and bottom panel: enteroendocrine cells (ChgA, green). Nuclei are stained with DAPI (blue), Scale bar = 50 μm. (C) Quantification of cell type abundance from two independent experiments. Data represent mean ± SD with each experiment including the three infant (in teal) and three adult (in purple) HIE lines. The P-values were calculated by one-way ANOVA. The asterisks (*** and ****) represent P < 0.001 and P < 0.0001, respectively.