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. 2000 Dec;74(23):11311–11321. doi: 10.1128/jvi.74.23.11311-11321.2000

FIG. 4.

FIG. 4

Southern analysis of recombinant VZVs. (A) Ethidium bromide-stained gel and Southern analysis of EcoRV-digested DNA prepared from Vero cells infected with either Oka-ATCC, Oka-R, or the three recombinant viruses Oka-gB2, Oka-gB3, and Oka-Δ4-3. Positions of molecular weight markers are shown in kilobases at the left. (B) Diagram depicting the ORF S/L region from the Oka-ATCC, Oka-Δ4-3, Oka-gB2, and Oka-gB3 viruses. Oka-ATCC is depicted on the top line, and the 1.2-kbp XhoI-to-PmeI probe is indicated. OkaΔ4 has a deletion of 400 nt between the ApaLI and BspEI sites (▵); and the gB mutants have a 60-nt CMV gB sequence (Inline graphic) containing a novel EcoRV site replacing the 120 bp between the EcoRI and BspEI sites of Oka. Images of the autoradiograms and stained gels were generated as for Fig. 3.