Fig. 3.

Conservative and expression analysis of Metallothionein 1E. A Agarose gel electrophoresis of polymerase chain reaction amplification fragment. Polymerase chain reaction fragment is 156 bp and contains the Metallothionein 1E c.G107A mutation site. B Sanger sequencing confirms the mutation. Polymerase chain reaction fragments are sequenced by Sanger, and the mutation is consistent with whole exome sequencing sequencing. C Metallothionein 1E p.C36 is conserved in different species such as human, macaque, chimpanzee, dog, and mice. D Analysis of Metallothionein 1E expression in breast cancer and para-cancer tissues in the Cancer Genome Atlas database. Red represents breast cancer, gray represents normal tissue; BRCA breast cancer