Fig. 1.

Expression of SOX4 mRNA in cancers. (A) Differential expression of sex-determining region Y-box (SOX4) mRNA in different human cancers and specific cancer subtypes from The Cancer Genome Atlas (TCGA) database in Tumor Immune Estimation Resource (TIMER). (B) High or low SOX4 expression in HCC and normal tissues in the UALCAN database. (C–F) SOX4 expression in HCC and adjacent noncancer tissues from the GSE36776, GSE60502, GSE84402, and GSE62232 datasets from the Gene Expression Omnibus database. (G) Representative images of liver in a liver cancer model in mice; Representative images of HE staining of liver tissue from mice; Immunohistochemical staining for SOX4 of liver tissue from mice. (H) SOX4 mRNA levels in different liver cancer cell lines. (I, J) Protein expression level of SOX4 in different liver cancer cell lines by Western blotting. After transferring the proteins and confirming the quality of the blots, we performed a precise cutting of the nitrocellulose membranes by comparing the molecular weights and electrophoretic migration rates of known proteins, allowing for subsequent quantitative analysis. Each experiment was repeated independently at least thrice. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS: not significant