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. 2024 Aug 14;23:92. doi: 10.1186/s12937-024-00990-w

Table 2.

Results of pairwise and dose–response meta-analysis

Group Study number; Patient number Dose–response meta-analysis, RR (95% CI) Pairwise Meta-analysis, RR (95% CI), NNT
400 IU/d 800 IU/d 1200 IU/d
Main analysis
 All [2870] 43; 49,320 0.99 (0.98–1.01) 0.99 (0.96–1.02) 0.99 (0.96–1.02) 0.99 (0.97–1.01)
Subgroup analysis
 Age group (years)
  < 7 [30, 32, 37, 39, 42, 51, 52, 54, 56, 60, 67, 69] 12; 8826 0.95 (0.87–1.03) 0.93 (0.83–1.04) 0.93 (0.84–1.03) 0.97 (0.91–1.13)
  7–17 [31, 33, 35, 44, 45, 55, 61, 64, 65] 9; 11,525 NA NA NA 1.00 (0.96–1.04)
  18–65 [28, 29, 34, 38, 40, 41, 46, 47, 49, 50, 57, 59, 70] 13; 3891 0.97 (0.91–1.03) 0.95 (0.86–1.05) 0.93 (0.84–1.04) 0.94 (0.86–1.02)
  > 65 [36, 43, 48, 53, 58, 62, 63, 66, 68] 9; 25,078 0.99 (0.97–1.01) 0.98 (0.95–1.01) 0.98 (0.94–1.01) 0.99 (0.98–1.01)
 Gender proportion (%)
  Male > 60 [29, 33, 36, 41, 44, 45, 51, 67] 8; 1930 0.92 (0.74–1.13) 0.93 (0.77–1.13) 0.95 (0.80–1.12) 0.96 (0.82–1.13)
  Male ≤ 60 [28, 3032, 34, 35, 3740, 42, 43, 4650, 5266, 6870] 35; 47,390 0.99 (0.99–1.00) 0.99 (0.98–1.00) 0.99 (0.98–1.00) 0.99 (0.96–1.01)
 Comorbidity
  General [28, 29, 31, 35, 37, 38, 4145, 48, 49, 5254, 5658, 6066, 6870] 29; 43,560 1.00 (0.99–1.01) 0.99 (0.98–1.01) 0.99 (0.98–1.01) 0.99 (0.97–1.01)
  Disease-specific [30, 3234, 36, 39, 40, 46, 47, 50, 51, 55, 59, 67] 14; 5610 0.94 (0.88–1.01) 0.92 (0.83–1.01) 0.91 (0.81–1.02) 0.97 (0.91–1.03)
 Baseline 25-hydroxyvitamin D levels (nmol/L)
  < 50 [35, 36, 43, 4648, 50, 54, 55, 57, 64, 70] 12; 11,588 0.99 (0.98–1.01) 0.99 (0.96–1.02) 0.99 (0.96–1.02) 0.98 (0.94–1.03)
  > 50 [28, 29, 33, 34, 3840, 42, 45, 49, 52, 53, 56, 5863, 65, 66] 21; 13,995 0.99 (0.98–1.01) 0.99 (0.96–1.02) 0.99 (0.96–1.02) 0.98 (0.95–1.02)
 Dosing frequency
  Bolus [30, 51, 67] 3; 1087 NA NA NA 0.96 (0.75–1.24)
  Daily [28, 29, 31, 3335, 39, 40, 42, 44, 45, 50, 52, 54, 5660, 62, 65, 69, 70] 23; 8788 0.94 (0.87–1.02) 0.92 (0.82–1.02) 0.92 (0.84–1.02) 0.92 (0.85–0.99), 36
  Weekly [32, 41, 49, 61, 64] 5; 12,864 NA NA NA 1.00 (0.98–1.02)
  Monthly [3638, 43, 4648, 53, 55, 63, 66, 68] 12; 26,581 0.99 (0.98–1.00) 0.99 (0.97–1.00) 0.98 (0.96–1.00) 1.00 (0.99–1.01)
 Trial duration (months)
  < 4 [28, 30, 35, 41, 44, 45, 57, 58, 69, 70] 10; 2845 0.86 (0.69–1.07) 0.80 (0.60–1.07) 0.81 (0.63–1.04) 0.81 (0.67–0.97), 16
  4–12 [29, 3133, 39, 42, 4952, 5961, 65, 67] 15; 6698 0.92 (0.85–1.01) 0.91 (0.81–1.02) 0.93 (0.83–1.03) 0.97 (0.89–1.05)
  > 12 [34, 3638, 40, 43, 4648, 5356, 6264, 66, 68] 18; 39,777 NA NA NA 1.00 (0.99–1.01)
 Climatic zone
  Tropical or Subtropical [61, 65, 67, 69, 70] 5; 2464 1.13 (1.00–1.29) 1.06 (0.97–1.16) 0.97 (0.82–1.15) 0.97 (0.77–1.21)
  Temperate [2860, 6264, 66, 68] 38; 46,856 0.99 (0.99–1.00) 0.99 (0.98–1.00) 0.99 (0.97–1.00) 0.99 (0.97–1.01)
 Summer
  Summer-inclusive [32, 34, 3638, 40, 42, 43, 4649, 51, 52, 5459, 6270] 29; 44,896 1.01 (0.99–1.02) 1.01 (0.98–1.04) 1.01 (0.98–1.03) 1.00 (0.98–1.02)
  Summer-sparing [2831, 33, 35, 39, 41, 44, 45, 50, 53, 60, 61] 14; 4424 0.83 (0.75–0.92) 0.77 (0.67–0.88) 0.79 (0.69–0.90) 0.85 (0.74–0.98), 26
 Winter
  Winter-dominant [2831, 35, 39, 44, 45, 60] 9; 1961 0.72 (0.62–0.82) 0.70 (0.61–0.81) 0.80 (0.71–0.90) 0.79 (0.71–0.89), 10
  Winter-non-dominant [3234, 3638, 4043, 4659, 6170] 34; 47,359 1.00 (0.99–1.02) 1.01 (0.98–1.03) 1.00 (0.98–1.03) 1.00 (0.98–1.02)
Sensitivity analysis
 Type of ARIs
  Mixed upper and lower respiratory tract infections [29, 3235, 42, 48, 49, 5356, 60, 6267] 19; 21,974 1.00 (0.98–1.03) 1.01 (0.97–1.05) 1.01 (0.97–1.06) 1.00 (0.97–1.03)
  Upper respiratory tract infections [28, 3841, 43, 4547, 50, 52, 54, 5759, 61, 68] 17; 22,395 0.99 (0.94–1.00) 0.99 (0.91–1.00) 0.99 (0.92–1.00) 0.98 (0.96–1.01)
  Lower respiratory tract infections [30, 36, 37, 51, 54] 5; 4305 0.98 (0.80–1.12) 0.95 (0.82–1.09) 0.95 (0.79–1.13) 0.95 (0.83–1.08)
  Influenza [31, 44, 52, 53, 55, 58, 59, 61, 69] 9; 3594 0.98 (0.88–1.08) 0.96 (0.80–1.13) 0.96 (0.80–1.15) 0.98 (0.89–1.07)

The dose–response meta-analysis could not achieve convergence on age group: 7–17 years, dosing frequency: weekly, and trial duration: > 12 months. The sample size was too small for dosing frequency: bolus to perform dose–response analysis. NNT was calculated for those meta-analyses showing significant preventive results

ARI Acute respiratory infection, NA Not available, NNT Number needed to treat