Table 1.

Primers and sequences used for COI and cytb conventional PCR, and real time RT-PCR methods

Primer	Sequence (direction, target gene)
VF1d_t1	5′-TGTAAAACGACGGCCAGTTCTCAACCAACCACAARGAYATYGG-3′ (forward, vertebrate COI)
VR1d_dt	5′-CAGGAAACAGCTATGACTAGACTTCTGGGTGGCCRAARAAYCA-3′ (reverse, vertebrate COI)
L14841	5′-CCATCCAACATCTCAGCATGATG AAA-3′ (forward, vertebrate cytb)
H151494	5′-GCCCCTCAGAATGA TATTTGTCCTCA-3′ (reverse, vertebrate, cytb)
LCO1490	5′-GGTCAACAAATCATAAAGATATTGG-3′ (forward, mosquitoes COI)
HCO2198	5′-TAAACTTCAGGGTGACCAAAAAATCA-3′ (reverse, mosquitoes COI)
Sense	5′-AAAGGAACAATGGACTCTGGTCA-3′ (forward, mosquitoes G2)
Antisense	5′-CACTTCTTACTACCATGTCCTCCAAT-3′ (reverse, mosquitoes G2)
Probe	5′-AAAGCTTTGATATCTCTCAGTGCCCCAA-3′ (5′-nuclease probe)