Fig. 2.

Immunohistochemical assessment of CD47 expression in ovarian carcinomas at diagnosis and after neoadjuvant chemotherapy. CD47 expression was assessed by IHC in paired samples at diagnosis (baseline, green point) and after neoadjuvant chemotherapy (NACT) (orange triangle) (n = 65). The quantification was conducted using the H-score system, combining staining intensity (0, + 1, + 2, + 3) with the percentage of positive cells (0 to 100) for a score of 0 to 300. a Lines connect samples from the same patient and indicate an increase (red) or decrease (green) in CD47 expression (left panel). Histograms showing quantification of CD47 expression (right panel). Statistical significance is indicated as **p < 0.01 (two-tailed Mann–Whitney test). b Representative images displaying representative CD47 staining patterns in three tumors from OC patients before and after NACT