Fig. 3.

(previous page). Correlation of CD47 with immune features after NACT. Tumor samples were collected from 101 patients from the CHIVA trial. a At baseline, we defined a CD47^highest (fourth quartile, score above 240) and a CD47^lowest population (first quartile, score under 150). Tumor samples were collected from 101 patients from the CHIVA trial. CD4⁺, CD8⁺, CD68⁺, CD163⁺, and FoxP3⁺ cells were assessed by immunofluorescence and scored as the number of positive cells. A mean score was calculated from three TMA cores from each sample. b CD8 (green point) and CD4 (blue square) staining were quantified at baseline (upper panels) or post NACT (lower panels) in the CD47^lowest (left panels) and CD47^highest (right panels) populations. The Pearson correlation coefficient (r) is indicated on each panel. The p-value was determined using the Wald test. c, Histogram representing CD4⁺ (blue), CD8⁺ (green), and FOXP3⁺ (pink) signal intensities at baseline (circle) and after NACT (triangle) in the CD47^lowest and CD47.^highest populations. Statistical significance is indicated as ns (not significant) or *p < 0.05 (one-tailed Mann–Whitney test)