Fig. 4.

Circulating TSP-1 is decreased after NACT. a Schematic illustration depicting the functional interaction between the CD47 receptor and the TSP-1 glycoprotein (left panel). The strategy employed to measure circulating TSP-1 levels in OC patients is depicted in the right panel. b Plasma levels of TSP-1 were determined using paired samples (n = 59) from the CHIVA cohort at baseline (green point) and post NACT (blue triangles). Connective lines (red for increased, green for decreased) link samples from the same patient to illustrate changes after treatment. Statistical significance is indicated by ****p < 0.0001 (two-tailed Mann–Whitney test). c Correlation plot displaying plasma TSP-1 levels versus tissue CD47 expression. d Kaplan–Meier survival curve representing patient overall survival (OS) based on circulating TSP-1 levels. The median was used as a cutoff to discriminate between low and high TSP-1 expression