FIG. 3.

Interaction of IE63 with p32 Sepharose column. HSV-1 wt- or 27-lacZ-infected, pCMV63-transfected, or mock-infected HeLa cell extracts were mixed with rMpGO-Sepharose or rMp32-Sepharose. After washing, proteins were boiled off the Sepharose, separated by SDS-PAGE, transferred to nitrocellulose, and Coomassie blue stained or analyzed by Western blotting using IE63 MAb. (A) Western blot using IE63 MAb of proteins from wt-infected (WT), 27-lacZ-infected, or mock-infected (MI) cell extracts, bound and then removed from an rMpGO (lanes 1 to 3) or rMp32 (lanes 4 to 6) column. (B) Coomassie blue staining of the bound proteins shown in panel A. (C) Western blot using IE63 MAb of proteins from wt-infected (WT), mock-infected (MI), or pCMV63-transfected (Trans) cell extracts, bound and then removed from an rMp32 column (lanes 5 to 8); cell extracts used (lanes 1 to 4).