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. 2024 Jul 8;15(8):e00039-24. doi: 10.1128/mbio.00039-24

Fig 5.

Venn diagram depicts the overlap of sulfated, sialylated, and fucosylated glycans. Scatterplot depicts the sum relative abundance of glycans pre-treatment and post-treatment. Line graphs plot changes in percentage relative abundance of glycans.

Fucosylated and sialylated glycans are degraded after growth of R. torques on cMUC2, but sulfated glycans accumulate. (A) Number and percentage of total glycans detected in cMUC2 pre- and/or post-growth of R. torques displaying indicated structural features by LC-MS/MS. (B) Sum relative abundance of glycans and inferred peeling reaction products pre- or post-R. torques growth on cMUC2 with respective structural features. Statistical analysis was performed with paired, two-tailed t-tests between pre- and post-growth samples for each structural feature category. *P < 0.05, **P < 0.01, ***P < 0.001. (C) Relative abundance of glycans detected that were retained on the mucin polypeptide backbone and were significantly different in pre- or post-growth samples of R. torques on cMUC2. Red lines indicate glycans that were not detected in any of the pre-growth samples but were detected in the post-growth samples. Each point represents the average relative abundance of three samples. (D) Putative structures of select detected glycans. Numbers refer to the corresponding plot in panel C. Structures generated with DrawGlycan (44). F, fucosylated; ns, not significant; S, sulfated; Y, sialylated.