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. 2024 Jul 8;15(8):e00039-24. doi: 10.1128/mbio.00039-24

Fig 6.

Relative abundance of Rt and Bt over 1, 3, and 5 days in co-culture. Growth of Bt and Am on digested PGM are also depicted. Abundance of Am and Bt over 1, 3, and 5 days in co-culture, and Bt’s relative abundance are displayed.

R. torques generates monosaccharide and O-glycans products from PGM that Bacteroides thetaiotaomicron can utilize. (A and B) Relative abundance of R. torques and B. thetaiotaomicron tdk−/− or 11× mutant in co-culture grown in partially DM and glucose (A, 5 mg/mL) or PGM (B, 10 mg/mL) calculated by quantitative PCR. Statistics were analyzed with two-tailed, unpaired t-tests comparing relative abundance of R. torques vs. B. thetaiotaomicron tdk−/− co-culture to relative abundances in R. torques vs. B. thetaiotaomicron 11× mutant co-culture at each respective timepoint. *P < 0.05, ****P < 0.0001; n = 9. (C and D) Growth of B. thetaiotaomicron (tdk−/−, 11× mutant, C) and A. muciniphila (D) on PGM pre-digested by R. torques supernatant for 0 or 24 h. B. thetaiotaomicron was grown in Bacteroides minimal medium, and A. muciniphila was grown in chopped meat medium. n = 3; points represent mean ± standard deviation. (E) Relative abundance of A. muciniphila and B. thetaiotaomicron in co-culture grown in partially-defined DM medium with PGM (5 mg/mL). (F) Relative abundance of B. thetaiotaomicron in each experiment shown in panel E. DM, defined medium; ns, not significant.