Fig. 4. ANGPTL4 regulates the expression of PPARγ.

(A) Angptl4 and Pparγ mRNA levels from hypothalamic astrocytes of fed and fasted WT mice (n = 4 mice per group). (B) Quantification and representative blots (from the same film) showing the levels of PPARγ in MBH of Angptl4^GFAP mice and their littermate controls (n = 5 mice per group). (C) Angptl4 mRNA levels and (D) LPL activity in the hypothalamus, hippocampus, and cortex of Angptl4^GFAP mice and their controls (n > 7 mice per group). (E) Quantification and representative blots (from the same film) showing the levels of AMPK (AMP-activated protein kinase), pAMPK, ACC (acetyl-CoA carboxylase), pACC, pHSL (phosphorylated hormone-sensitive lipase), and CD36 in the MBH of Angptl4^GFAP mice and their controls (n = 5 mice per group). (F) Pparγ mRNA levels from hypothalamic astrocytes of Ppar^GFAP mice and their controls (n = 4 samples per group). (G) Cd36, Srebp, and Acc mRNA levels from hypothalamic astrocytes of Ppar^GFAP and their control mice (n = 4 samples per group). a.u., arbitrary units. (H) Cd36, Srebp, Acc, and Fasn mRNA levels from primary cultures of hypothalamic astrocytes of Ppar^GFAP and their control mice treated with bovine serum albumin (BSA) or free fatty acid (FFA) (n = 4 or 5 samples per group). Data are presented as means ± SEM. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 as determined by two-tailed t test.