Fig 7.

Addition of MPA shortly before or after confluence results in rapid, guanine-sensitive Imd2-GFP induction. Top panel: population mean Imd2-GFP fold induction (solid lines) with one standard deviation above and below the mean (shaded area) for cells perfused for 10 or 20 hours with untreated media before addition of 1.5 µg/mL MPA without or with 400 µM guanine. Colored arrows indicate the time that MPA or MPA + guanine was added to the cells (see key). Bottom panel: the percentage of the viewing field occupied by cells over time.