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. 2024 Jul 16;15(8):e01420-24. doi: 10.1128/mbio.01420-24

Fig 3.

TEM micrographs of NU J and Rag1ko feature viral particles, with more particles for Rag. IHC micrographs of NU J and Rag1ko feature MmuPV1 L1 capsid protein higher in Rag1ko, and Western Blot features a band stronger in Rag1ko.

Viral particles were found in the cervicovaginal swabs of infected mice. Cervicovaginal swabs were collected from both NU/J heterozygous (A) and Rag1ko (B) infected mice and tested for viral particle presence by TEM (scale bar 100 nm). Consistent with previous reports, viral particles around 50 nm in diameter were found in both mouse strains. Rag1ko (B) mice produced more viral particles in the cervicovaginal swab samples when compared to those in NU/J heterozygous mice (A). Higher numbers of viral capsid protein L1 were detected in the infected vaginal tissues of Rag1ko (20×, D) mice than those in NU/J heterozygous (C) mice by IHC using in-house monoclonal antibody MPV.B9 (20×, Red dots, arrows). MmuPV1 L1 was detected using an in-house monoclonal antibody MPV.B9 via western blot (E).