FIG. 1.

(A) Schematic representation of ΔMP:GFP constructs used in this study. MP and GFP sequences are represented by dark grey and white, dashed bars, respectively. MP:GFP (24) is shown at the top, and ΔMP:GFP derivatives are depicted below. The ΔMP:GFP derivatives include mutants with up to 81 amino acids deleted from the C terminus (C35, C55, C66, and C81) and 3 or 28 amino acids deleted from the N terminus (the first two amino acids, N5 and N30, were not deleted) of MP. (B and C) Immunoblot analysis of infected tobacco BY-2 protoplasts expressing wild-type MP:GFP and ΔMP:GFP mutants with antibody directed against amino acids 6 to 22 (B) and 209 to 222 (C) of MP. Mk, mock-infected protoplasts; M, protein size markers (positions are indicated in kilodaltons).