FIG. 7.

hTfR expression and transduction of T24 cells by hTfR-targeting epitope modified adenoviruses. (A) Immunostaining for hTfR expression. T24 cells were fixed and stained with monoclonal antibody 128.1, followed by rhodamine-conjugated goat anti-mouse antibodies. The photomicrograph is representative of the entire plate. (B) T24 cells (1 × 10⁵) were transduced with Ad5GFPB5HI, Ad5GFPB6HI, etc., or Ad5GFP (4 × 10⁸ particles), and GFP-positive cells were quantitated by FACS. For blocking experiments T24 cells, were incubated with human soluble hTfR or transferrin for 30 min at 4°C prior to the addition of virus. The various viruses are indicated by the hTfR-targeting epitope (B5, B6, etc.). “C” corresponds to Ad5GFP. The data are the means ± the SEM and are from three independent experiments.