TABLE 2.
Peptide motifs and their effects on recombinant adenovirus production
| Peptide | Epitope | Virus productiona |
|---|---|---|
| Control | ++++ | |
| IEAYAKKRK | B1 | ++ |
| GHKVKRPKG | B2 | ± |
| KDKIKMDKK | B3 | ++++ |
| G KNKIPKSPK LGSb | B4 | − |
| G GKGPKWMR LGS | B5 | +++ |
| G GHKAKGPRK LGS | B6 | ++++ |
| G VIAKIKKPK LGS | B7 | +++ |
| G KWKTPKVRV LGS | B8 | + |
| G LQAKKKRPK LGS | B9 | − |
| G VEAKGHKKK LGS | B10 | − |
a
“++++” indicates virus production characteristic of Ad5GFP. “−” indicates that the virus could not be amplified to reasonable titers.
b
Motifs B4 through B10 included an amino G and a carboxyl LGS linker.