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. 2024 Jun 13;134(16):e178692. doi: 10.1172/JCI178692

Figure 3. Increased constitutive and activation-induced TCR signaling in patients.

Figure 3

(A) Immunoblot analysis of unstimulated PBMCs from healthy controls and patients. (B) Representative immunoblots of LCK and ZAP70 phosphorylation in T cell blasts derived from 1 healthy control and 1 patient (left) with or without anti-CD3/anti-CD28 (α-CD3/CD28) stimulation for 15 minutes. Summary results (right) are from 3 experiments, each with 1 healthy control and 1 patient. (C) p-ERK was measured by flow cytometry in T cell blasts derived from healthy controls (n = 3) and patients (n = 3) after anti-CD3/anti-CD28 stimulation for 15 minutes. (D and E) CD69 (D) and CD25 (E) expression was measured by flow cytometry on T cell blasts derived from healthy controls (n = 3) and patients (n = 3) after anti-CD3/anti-CD28 stimulation for 24 hours. Unstim., unstimulated. (F) Immunoblot analysis of NUR77 levels in T cell blasts derived from healthy controls and patients after anti-CD3/anti-CD28 stimulation for 24 hours. Data represent the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001, by 2-tailed, unpaired (A and C–F) or 2-tailed, paired (B) Student’s t test.