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. 2024 Aug 7;632(8025):647–655. doi: 10.1038/s41586-024-07726-0

Extended Data Fig. 4. STED super-resolution imaging of the apical interface.

Extended Data Fig. 4

(a) Combining 3D tissue culture of MDCK-II cysts with STED microscopy enables super- resolution imaging of cell-cell interfaces. At the equator cell-cell interfaces are oriented parallel to the high-resolution axis (XY) of the microscope. (b) 2-color STED imaging of PATJ (yellow) and ZO-1 (magenta) in MDCK-II cysts reveal that ZO-1 forms a network structure reminiscent of tight junction strands. PATJ is enriched as clusters at the apical interface of the ZO-1 network.