TABLE 1.
Genetic and phenotypic properties of parental and mutant Sindbis viruses
| Virus | Mutation(s) relative to TRSB | Glycoprotein compositiona | Cell line used for selection of mutant |
|---|---|---|---|
| Parental | |||
| TRSB (wild type) | E1/E2 | ||
| TRSB-N (non-infectious)b | E2 residue 1 Arg to Asnc | E1/PE2 | |
| Infectious mutants | |||
| NE2G216 | E2 residue 1 Arg to Asn | E1/PE2 | BHK-21 |
| E2 residue 216 Glu to Glyd | |||
| N6R1 | E2 residue 1 Arg to Asn | E1/PE2 | BHK-21 |
| E3 residue 25 Cys to Argd | |||
| NS-4 | E2 residue 1 Arg to Asn | E1/PE2 | C6/36 |
| E3 residue 34 Leu to Hisd | |||
| NS-7 | E2 residue 1 Arg to Asn | E1/PE2 | C6/36 |
| E3 residue 46 Tyr to Hisd |
a
As determined by electrophoretic analysis of purified virions.
b
For a description of this virus, see reference 8.
c
This mutation generates a signal for N-linked glycosylation at E2 residue 1 and is individually lethal.
d
This mutation suppresses the lethal effects of oligosaccharide addition at E2 residue 1.