TABLE 2.
Effects of 1-dMM on virus replication in Pro-5 cells
| Virus sample | Virus titera (PFU/ml) in the absence of 1-dMM | Ratiob | Virus titer (PFU/ml) in the presence of 2.5 mM 1-dMM | Ratio |
|---|---|---|---|---|
| TRSB | ||||
| Culture medium and cellsc | 6.3 × 108 | 4.2 | 1.1 × 109 | 4.4 |
| Culture mediumd | 1.5 × 108 | 2.5 × 108 | ||
| NE2G216 | ||||
| Culture medium and cells | 4.7 × 107 | 2.8 | 3.1 × 107 | 397 |
| Culture medium | 1.7 × 107 | 7.8 × 104 |
a
Virus titers were determined by plaque assay on monolayers of BHK-21 cells.
b
Virus titer obtained for culture medium and cells divided by the virus titer obtained for culture medium only.
c
Cells were scraped from dishes and collected together with culture medium, then frozen at −70°C to lyse the cells.
d
Sample of culture medium clarified of cells by microcentrifugation.