FIGURE 3.

H₂S inhibited ACR-induced ferroptosis in Sertoli cells. (A) Reversion of GSH level by NaHS. TM4 cells were exposed to 100 µM ACR with or without 1 mM NaHS for 3 h. The cellular lysates were analyzed for GSH levels with GSSG/GSH quantification kit. Data are mean ± S.E. (n = 4; *P < 0.05, **P < 0.01). (B,C) Upregulation of SLC7A11 by NaHS. TM4 cells were exposed to 100 µM ACR with or without 1 mM NaHS for 3 h. Cellular lysates were used to analysis of protein expression of SLC7A11 (B). Densitometric quantitation of these bands was shown in (C). Data in (C) are mean ± S.E. (n = 3; *P < 0.05, **P < 0.01). (D,E) Clearance of Fe²⁺ accumulation by NaHS. TM4 cells were stimulated with the indicated concentrations of ACR with or without NaHS for 3 h. FerroOrange staining was performed for Fe²⁺ detection. (F,G) Prevention of lipid peroxidation by NaHS. TM4 cells were exposed to 100 µM ACR with or without NaHS for 3 h. After that, C11 BODIPY 581/591 staining were used for indication of lipid oxidation. Densitometric quantitation of relative fluorescence intensity in (D,F) was performed on 20 cells randomly selected from each group, and the data were shown in (E,G), respectively (n = 20; **P < 0.01).