Fig. 2.

Percentage of ¹²⁵I-T4 uptake compared to the vehicle control 0.1% DMSO in A CHO-K1 OATP1C1 and B MDCK-OAT4 cells in the presence of selected chemicals. CHO-K1 OATP1C1 and MDCK-OAT4 cells were exposed to 10 nM T4 and 10–100 µM of selected chemicals for 30 min and 20 min respectively. Chemicals that decreased T4 uptake below 80% (i.e. 20% inhibition) after exposure were considered positive hits. 0.1% DMSO was used as vehicle control, 100 µM ICG was used as a positive control for OATP1C1 and 100 µM LESI for OAT4 (in black). Results are shown as mean ± SD, N = 3. Comparisons with a p values below 0.05 are indicated by a star