Table 2.

Inhibition of T4 uptake and cytotoxicity of individual compounds in the CHO-K1 OATP1C1 TH uptake assay

Substance (by order of BMC20)		Concentration response function					T4 uptake inhibition	Cytotoxicity
	Nstudy	RM	${\widehat{\theta }}_1$	${\widehat{\theta }}_2$	${\widehat{\theta }}_{\mathit{min}}$	${\widehat{\theta }}_{\mathit{max}}$	BMC20 (M), [CI]	IC10 (M)
Tetrabromobisphenol A	4	Logit	− 23.30	− 2.82	0*	1.042	2.03E−09 [1.28E−09; 3.22E−09]	> 3.00E−07
Quercetin	3	Logit	− 3.90	− 0.73	0*	0.974	3.94E−08 [4.41E−09; 3.51E−07]	> 1.00E−04
Pentachlorophenol	4	Logit	− 8.05	− 1.35	0*	0.977	8.81E−08 [5.80E−08; 1.34E−07]	> 1.85E−05
Indocyanine green	4	Logit	− 13.10	− 2.19	0*	1.084	3.60E−07 [2.17E−07; 5.97E−07]	> 1.00E−04
Perfluorooctanesulfonic acid	3	Logit	− 10.23	− 1.84	0*	0.976	4.03E−07 [2.32E−07; 7.00E−07]	> 1.00E−04
Lesinurad	3	Logit	− 9.22	− 1.71	0*	0.954	4.34E−07 [1.97E−07; 9.56E−07]	> 1.00E−04
Probenecid	3	Logit	− 4.42	− 0.97	0*	0.962	6.66E−07 [1.24E−07; 3.57E−06]	> 1.00E−04
Bromosulfophthalein	4	Logit	− 11.37	− 2.16	0*	0.946	8.72E−07 [5.70E−07; 1.33E−06]	> 1.00E−04
Diclofenac	3	Logit	− 6.56	− 1.31	0*	1.062	1.40E−06 [6.84E−07; 2.87E−06]	> 1.00E−04
Triclosan	3	Logit	− 28.80	− 5.33	0*	1.025	2.30E−06 [1.38E−06; 3.83E−06]	3.78E−07
Sulforhodamine 101	4	Logit	− 13.60	− 2.69	0*	1.011	2.86E−06 [1.29E−06; 6.34E−06]	> 1.00E−05
Perfluorooctanoic acid	3	Logit	− 7.08	− 1.50	0*	1.036	2.94E−06 [1.04E−06; 8.36E−06]	> 1.00E−04
Bisphenol AF	4	Logit	− 32.69	− 6.27	0*	0.975	3.52E−06 [2.03E−06; 6.11E−06]	9.09E−07
Silychristin	3	Logit	− 14.50	− 2.92	0*	1.005	3.78E−06 [1.54E−06; 9.27E−06]	> 1.00E−05
Verapamil	3	Logit	− 7.60	− 1.88	0*	1.010	1.74E−05 [6.34E−06; 4.79E−05]	> 1.00E−04
Bisphenol A	4	Logit	− 14.60	− 3.35	0*	1.042	1.90E−05 [1.28E−05; 2.83E−05]	4.34E−06
Bisphenol S	3	Logit	− 7.72	− 2.07	0*	0.972	3.38E−05 [7.87E−06; 1.45E−04]	> 1.00E−04
Rifampicin	3	Logit	− 19.48	− 4.69	0*	1.063	4.06E−05 [2.58E−05; 6.38E−05]	> 1.00E−04
Bisphenol F	3	Logit	− 14.10	− 3.56	0*	1.004	4.49E−05 [2.80E−05; 7.20E−05]	> 1.00E−04
Bisphenol Z	4	Linear	–	− 2293	–	1.009	n.d. [n.d.; n.d.]	> 1.00E−04

BMC20: concentration that inhibits the T3 uptake by 20%; IC10: concentration that inhibits cell viability by 10% in the CellTiter-Glo® luminescent cell viability assay. Values in brackets denote the upper and lower limits of the approximate 95% confidence interval (CI); the column “RM” indicates the mathematical regression function as defined at Scholze et al. (2001): ${\widehat{\theta }}_1,{\widehat{\theta }}_2,{\widehat{\theta }}_{\mathit{min}},{\widehat{\theta }}_{\mathit{max}}$ estimated model parameters, given for concentrations expressed in M (rounded values), with * highlighting parameters that were not estimated but constrained to a fixed value. N_study = number of independent experiments