Table 3.

Inhibition of uptake and cytotoxicity of individual compounds in the MDCK-OAT4 TH uptake assay

Substance (by order of BMC20)		Concentration response function					T4 uptake inhibition	Cytotoxicity
	Nstudy	RM	${\widehat{\theta }}_1$	${\widehat{\theta }}_2$	${\widehat{\theta }}_{\mathit{min}}$	${\widehat{\theta }}_{\mathit{max}}$	BMC20 (M), [CI]	IC10 (M)
Bromosulfophthalein	3	Logit	− 19.76	− 3.02	0.059	0.922	7.25E−08 [3.85E−08; 1.36E−07]	> 1.00E−04
Tetrabromobisphenol A	3	Logit	− 18.37	− 3.21	0	0.986	6.49E−07 [4.32E−07; 9.74E−07]	> 1.00E−05
Perfluorooctanoic acid	3	Logit	− 5.98	− 1.25	0	1.006	1.38E−06 [4.27E−07; 4.44E−06]	> 1.00E−04
Perfluorooctanesulfonic acid	3	Logit	− 13.81	− 2.69	0	0.920	1.44E−06 [4.08E−07; 5.05E−06]	> 1.00E−04
ß-Estrone-3-sulfate	2	Logit	− 5.89	− 1.21	0	1.056	1.55E−06 [5.32E−07; 4.51E−06]	> 1.00E−05
Diclofenac	3	Logit	− 11.41	− 2.35	0	0.980	3.22E−06 [6.39E−07; 1.62E−05]	> 1.00E−04
Lesinurad	3	Logit	− 13.72	− 2.74	0	1.016	3.23E−06 [2.26E−06; 4.61E−06]	> 1.00E−04
Bisphenol AF	3	Logit	− 10.62	− 2.32	0	0.966	5.39E−06 [1.65E−06; 1.76E−05]	9.69E−08
Rifampicin	3	Logit	− 13.49	− 2.96	0	1.021	1.02E−05 [4.85E−06; 2.14E−05]	> 1.00E−04
Quercetin	3	Logit	− 9.12	− 2.13	0	1.010	1.23E−05 [4.77E−06; 3.16E−05]	> 1.00E−04
Indocyanine green	3	Logit	− 17.36	− 3.91	0	1.017	1.68E−05 [1.20E−05; 2.33E−05]	> 1.00E−05
Pentachlorophenol	3	Logit	− 24.58	− 5.35	0	1.678	2.65E−05 [2.17E−05; 3.23E−05]	1.14E−06
Probenecid	3	Linear	–	− 4474	0	0.963	3.65E−05 [2.69E−05; 4.61E−05]	> 1.00E−04

BMC20: concentration that inhibits the T3 uptake by 20%; IC10: concentration that inhibits cell viability by 10% in the CellTiter-Glo® luminescent cell viability assay. Values in brackets denote the upper and lower limits of the approximate 95% confidence interval (CI); the column “RM” indicates the mathematical regression function as defined at Scholze et al. (2001): ${\widehat{\theta }}_1,{\widehat{\theta }}_2,{\widehat{\theta }}_{\mathit{min}},{\widehat{\theta }}_{\mathit{max}}$ estimated model parameters, given for concentrations expressed in M (rounded values), with * highlighting parameters that were not estimated but set to a fixed value. N_study = number of independent experiments