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Exemplary over- and underestimation of lacunar volume using [Eq. 1] on cross-sections of an artificial lacunae dataset. A: Different cutting planes result in drastically different over- or underestimation for both tested resolutions (0.2 μm, 1.45 μm). B: For realistic lacunar orientations in lamellar bone (0–22°) or randomly oriented lacunae, lacunar volume is always underestimated
