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. 2024 Aug 14;14:18851. doi: 10.1038/s41598-024-69261-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of different matrices on cell migration in HCT-116-GFP spheroids cultured for seven days. (a) Representative images of non-embedded and embedded HCT-116-GFP spheroids in different matrices. Upper panel shows the whole spheroid; lower panel shows zoomed-in section. Scale bar: 200 µm. (b) Analysis of mRNA expression of Snail and MMP9 in non-embedded and embedded HCT-116-GFP spheroids in different matrices. NES: non-embedded spheroids, M: Matrigel^®, C: collagen I and MC: mixture of Matrigel^® and collagen I. n = 3. n.d. = not detected (ct-value > 35.00). Data are shown as mean ± SD (*p < 0.05).