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. 2024 Aug 14;14:18851. doi: 10.1038/s41598-024-69261-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Investigation of migration for HCT-116-GFP in collagen I matrix in the dynamic 3D cell culture system. (a) Experimental workflow of spheroid generation by liquid overlay technique, embedding in collagen I matrix and analysis through microscopy and gene expression. (b) Representative images of spheroids cultured in liquid overlay (non-embedded (NES)), static or dynamic conditions illustrating the distinct core (dotted outline) and migration areas used for analysis. (c) Representative images of embedded spheroids in static and dynamic culture conditions. Quantification of spheroid migration on day 3 and day 7 (d), n = 8. Scale bar: 200 µm. Data are shown as mean ± SD (*p < 0.05).