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. 2024 Aug 14;14:18851. doi: 10.1038/s41598-024-69261-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histology staining of HCT-116-GFP spheroids in non-embedded spheroids (NES), static and dynamic culture conditions. (a) H&E staining. (b) TUNEL assay. (c) Immunohistochemistry with specific antibody against pHH3, arrows indicate pHH3-positive cells. (d) Quantitative result of TUNEL assay, the number of TUNEL-positive cells (green) is normalized to the total nuclei (purple). (e) Quantitative result of pHH3 staining, the number of pHH3-positive cells is normalized to the total area of spheroids. Scale bar: 200 µm. Data are shown as mean ± SD. n = 4 (*p = < 0.05).