Heparan sulfate (HS) disaccharides analysis for striatum (ST).A, absolute abundance in fmol for seven HS disaccharides (D0A0, D2A0, D0A6, D0S0, D2S0, D0S6, and D2S6) (D2A6 was not detected) for saline (blue color), cocaine (red color), and METH (green color) in ST. B, total HS disaccharide abundance (fmol) for saline-, cocaine-, and METH-treated samples in ST. C, sulfate content (%) for saline-, cocaine-, and METH-treated samples in ST. D, N-acetylated and N-sulfated HS disaccharide abundance (fmol) for saline-, cocaine-, and METH-treated samples in ST. (∗=p ≥ 0.05, ∗∗= p ≥ 0.001, ∗∗∗= p ≥ 0.0001, ∗∗∗∗= p ≥ 0.00001) (mean ± SD). E, unsupervised hierarchical clustering of the entire HS disaccharide data for saline, cocaine, and METH samples. F, heatmap representation of relative quantification for HS disaccharides (red: increased and green: decreased abundance). METH, methamphetamine.