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Comparison between EPE values under optimal conditions applying ultrasound-assisted extraction (UAE) or freeze-thaw (FT) methods for cell disruption combined with different aqueous extraction mixtures (IL: ionic liquid mixture optimised in this work, PB: 0.1 M phosphate buffer at pH 7.0; AB: 0.1 M acetate buffer at pH 5.2; BB: 0.1 M borate buffer at pH 9.0). All experiments were performed at 35 °C for 30 min, using a biomass concentration of 10 mg mL−1.
